*Scoring is available for 20-nt type II sgRNAs. See Doench et al., 2014 for details.
High scores may indicate more potent sgRNAs. However, some potent sgRNAs have scores < 0.1 and a number of successful sgRNAs had scores between 0.1 and 0.2.
Therefore, potential experimental utility and subsequent experimental verification should guide the choice of sgRNAs.
Off-target analysis parameters: GT-Scan: No restrictions on PAM sequence or target length. Adjust the target rule on the submission form.Cas-OFFinder: Length should be 20bp for SpCas9 (PAM = 'NGG'), 18bp for StCas9 (PAM = 'NNAGAAW'), and 24bp for NmCas9 (PAM = 'NNNNGMTT').
*Refseq sequences can be from any species
*The program supports Ensembl gene and transcript identifiers, gene symbols as well as Refseq cDNA accession numbers (if available).
Small number of identifiers (2-3) is most suitable for this application unless similarity is expected to be high.
CRISPR MultiTargeter was developed by Sergey Prykhozhij at the IWK Health Centre and Dalhousie University. The design of the logo and the buttons was by Vinothkumar Rajan. The latest update was in May 2019.